Figure 3 Figure 3. A murine protein-protein interaction (PPI) network for signaling proteins assessed from the regulation of gene expressions. For A) & B) the network contains 281 murine proteins linked by 551 unique interactions. Blue nodes represent proteins that are in the network but are not regulated by the 56Fe irradiation perturbation, red nodes are determined to be up-regulated, and green nodes are down-regulated. A) PPI network showing regulations of tumors growing in young 56Fe-irradiated mice as compared with those in young unirradiated mice. B) PPI network regulations in tumors growing in middle-age 56Fe-irradiated mice compared with those in middle-aged unirradiated mice. For (C) – (F) pathway analysis was done with Ingenuity Pathway Analysis (IPA) software. Network depicted contains central nodes from AKT1 and FASN with direct (— solid lines) and indirect (- - dashed lines) relations to these molecules. Log2 fold changes to the gene expression were used to obtain different shades of green for regulation levels for down-regulated genes, while different shades of red depict regulation levels for up-regulated genes. Grey genes exist in the network without a significant 2-fold change under the perturbation investigated. The darker the shade of green or red, the greater the fold change.

Reference: Age and space irradiation modulate tumor progression: implications for carcinogenesis risk. Beheshti A, Sachs RK, Peluso M, Rietman E, Hahnfeldt P, Hlatky L. Radiat Res. 2013 Feb;179(2):208-20. Epub 2013 Jan 4.]

Close this window (or tab) to return to the Center of Cancer Systems Biology at GRI/Tufts website.